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Study on Optimum Regeneration System in Phalaenopsis

Wenhua Xu

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Abstract

The experiment in the technology and system of tissue culture of Phalaenopsis was carried out. The results showed that the suitable induction medium for the PLBS(protocorm-like body of Phalaenopsis) was MS+ 6-BA 6 mg/L+ NAA 1 mg/L+ Ad 10 mg/L+ sugar 20 g/L+ coconut juice 20 % + agar 7.5 g/L (pH 5.4). The suitable propagation medium for the PLBS was MS + 6-BA 2 mg/L+ NAA 1 mg/L + Ad 10 mg/L+ sugar 20 g/L +coconut juice 20 %+agar 7.5 g/L (pH 5.4). The suitable regeneration medium was MS+NAA 1 mg/L + sugar 20 g/L+ active carbon 1 g/L+ banana mire 100 g/L+malic acid 30 mg/L+agar 7.5 g/L (pH 5.4).

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What this paper is about

The experiment in the technology and system of tissue culture of Phalaenopsis was carried out. The results showed that the suitable induction medium for the PLBS(protocorm-like body of Phalaenopsis) was MS+ 6-BA 6 mg/L+ NAA 1 mg/L+ Ad 10 mg/L+ sugar 20 g/L+ coconut juice 20 % + agar 7.5 g/L (pH 5.4). The suitable propagation medium for the PLBS was MS + 6-BA 2 mg/L+ NAA 1 mg/L + Ad 10 mg/L+ sugar 20 g/L +coconut juice 20 %+agar 7.5 g/L (pH 5.4). The suitable regeneration medium was MS+NAA 1 mg/L + sugar 20 g/L+ active carbon 1 g/L+ banana mire 100 g/L+malic acid 30 mg/L+agar 7.5 g/L (pH 5.4).

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Available abstract

The experiment in the technology and system of tissue culture of Phalaenopsis was carried out. The results showed that the suitable induction medium for the PLBS(protocorm-like body of Phalaenopsis) was MS+ 6-BA 6 mg/L+ NAA 1 mg/L+ Ad 10 mg/L+ sugar 20 g/L+ coconut juice 20 % + agar 7.5 g/L (pH 5.4). The suitable propagation medium for the PLBS was MS + 6-BA 2 mg/L+ NAA 1 mg/L + Ad 10 mg/L+ sugar 20 g/L +coconut juice 20 %+agar 7.5 g/L (pH 5.4). The suitable regeneration medium was MS+NAA 1 mg/L + sugar 20 g/L+ active carbon 1 g/L+ banana mire 100 g/L+malic acid 30 mg/L+agar 7.5 g/L (pH 5.4).

Key concepts: Phalaenopsis, Sugar, Agar, Malic acid, Chemistry, Food science, Botany, Biology

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