Isolation and screening of cellulose-decomposing microorganisms

The 39 strains of cellulose-decomposing microorganisms were isolated from rubbish compost by Dubos liquid medium with Xinhua filter paper as the sole carbon source.We got 10 colonies surrounded by some distinct zones of clearing in cellulose-congo red agar medium from the 39 strains above.By measuring the enzyme activity of liquid culture filtrates,we got a strain(termed B-6)with higher cellulase activity.In order to evaluate our screening,the results of liquid and solid fermentation were compared between strain B-6 and AS33 711.We found out that they had similar enzyme-producing property.The enzyme activity of B-6 reached 39.2 and 14.9units,comparied to AS3.3711 with 16.6 and 15.7 units in solid and liquid fermentation respectively.In addition,B-6 had higher capacity of liquefying CMC.It could liquidize 3% CMC completely within 24 hours,while AS3.3711 needed 96h to do it.