Research on the Screening and Mixed Fermentation of Cellulose-decomposing Microorganisms

[Objective] The aim of the research was to supply theoretical basis for high efficient decomposition of cellulose.[Method] The strains that could decompose cellulose better were screened out from samples containing lignocellulose-decomposing microorganisms,such as forest defoliation soil,rotten straw and farmhouse compost.They were cultured solely and by mixed fermentation.[Result] Four strains that could decompose cellulose better were screened out finally,and they were judged as 3 strains of bacteria and 1 strain of actinomycete preliminarily.The mixed culture of strains enhanced cellulase activity to some degree,the enzyme activity of strain combination D6/D7 reached 67.12 U in 72 h and was equal to 2 times of that in its sole culture.The variant curve of cellulase activity in most strains varying with time showed the ascendingdescending-ascending trend,but the stability of strain combination D6/D7 was better.[Conclusion] The mixed culture of strains could enhance cellulase activity,especially for strain combination D6/D7.