Study on Isolation of Cellulose-decomposing Microorganisms and its Cellulase-producing Conditions

[Objective] The aim of this research was to seek for the optimum cellulase-producing conditions for cellulose-decomposing microorganisms.[Method] In the strain screening and identifying tests,the effect of 5 kinds of C resources (bran,filter paper,glucose and CMC),5 kinds of N resources(peptone,ammonium nitrate,ammonium oxalate,ammonium nitrate and citric acid),culture time,initial pH of liquid fermentation medium and 5 culture temperatures(22,26,30,34 and 38 ℃) on producing cellulase by microorganisms.[Result] Seventeen strains of cellulose-decomposing microorganisms were isolated from cattle manures and soil,among which,T2 strain had dense mycelium,and its colony could grow faster on the PDA medium and it was identified as trichoderma.The optimum cellulase-producing conditions for T2 strain was follows: with the cellulose matters such as bran and CMC as the C resources and the peptone and ammonium nitrate as the N source,culture time of 3～4 h,the initial pH of liquid fermentation medium in 4～6 and culture temperature of 26～34 ℃.[Conclusion] When T2 strain was cultured at 30 ℃,the highest enzyme activities with the filter paper and CMC as C resources could go up to 10.72 and 47.52 U/g,resp.