Bacterial Distribution and Drug-resistance of Clinical Specimens

Objective To learn the distribution and drug-resistance trend of pathogenic bacteria in our hospital,in order to provide basis for correct use of clinical antimicrobial agents.Methods Between January 2011 and January 2012,we performed bacterial culture and drug sensitivity test for 1643 clinical specimens coming from both outpatients and inpatients,and analyzed drug resistance of the bacteria.Results Among the 1 643 bacterial culture specimens,we found a total of 750 strains of pathogenic bacteria,indicating a positive rate of 45.6%.Among the pathogenic bacteria,there were 242 strains of G+ cocci,occupying 32.3% with a majority of staphylococcus aureus,coagulase negative staphylococcus and enterococcus genera;there were 382 strains of G-bacilli,occupying 50.9% with a majority of escherichia coli,pseudomonas aeruginosa,klebsiella pneumoniae,enterobacter and acinetobacter baumannii.Methicillin resistant staphylococcus aureus(MRSA) accounted for 59.0% of staphylococcus aureus.And the drug resistance rate of staphylococcus aureus to penicillin and cefazolin were both 100.0%.Among the enterococcus,dung enterococcus and shit enterococcus were of the highest separation rate.The drug resistance rate of dung enterococcus to penicillin was 35.3%.The detection rates of escherichia coli and klebsiella pneumoniae producing ESBL(Extended-spectrum beta-lactamase) were 77.6% and 56.7% respectively;non-fermentative bacteria consisted mainly of pseudomonas aeruginosa and baumanii,and the separation rate of olefinic kind of acinetobacter baumannii resisting carbon blue mould from acinetobacter baumannii was 37%.The drug resistance rates of acinetobacter baumannii and pseudomonas aeruginosa to imipenem were 46.3% and 28.3% respectively.Conclusions In our hospital,the bacterial drug resistance is high.Reasonable application of antimicrobial agents should be strengthened;the use of the third generation cephalosporins and other beta lactam kind of antibacterial drugs should be careful in order to reduce antibiotic selective pressure to prevent drug-resistant strains within the hospital.