Distribution and drug susceptibility of the pathogenic bacteria isolated from clinical specimens

Objective To explore the distribution and drug resistance of clinical isolated pathogens so as to provide scientific basis for the correct diagnosis and rational use of antibiotics.Methods The isolation of pathogens was performed according to the National Clinical Laboratory Procedures.The identification and drug susceptibility of clinical isolates was carried out using the VITEK2 Compact system,and the results were determined based on the interpretation of CLSI 2012 break point.Results A total of 6 018 strains of pathogens were isolated from 20 319 specimens,among which Acinetobacter baumannii(19.14%),Escherichia coli(15.84%),Pseudomonas aeruginosa(14.57%),Klebsiella pneumoniae(14.29%),Staphylococcus aureus(5.62%)were the first five most common pathogens.The bacteria were mainly isolated from sputum(47.02%),other fluids(22.04%),blood(20.50%),urine(8.24%) and stool(2.20%).The methicillin-resistant Staphylococcus aureus,Staphylococcus epidermidis,and Staphylococcus haemolyticus were 34.91%,77.18% and 85.71%,respectively.No strains of vancomycin-,linezolid-and tigecycline-resistant Staphylococci,Enterococcus faeciumand Enterococcus faecalis were detected.The detection rates of the Escherichia coli,Klebsiella pneumoniae and Klebsiella oxytoca producing extendedβ-lactamases(ESBLs)were 60.76%,29.53% and 40.63%,respectively.Enterobacteriaceae remained at a high sensitivity to carbapenems,though imipenem-resistant Enterobacteriaceae have occurred. The non-fermenting bacteria were highly resistant to carbapenems.The imipenem-sensitive rates of Acinetobacter baumannii and Pseudomonas aeruginosa were 35.42% and 59.52%,respectively.Conclusion Acinetobacter baumannii,Escherichia coli,Pseudomonas aeruginosa,Klebsiella pneumonia and Staphylococcus aureus were the five most common pathogens.Bacterial resistance situation is still serious and more effective measures should be taken to control it.