Common clinical bacterial isolates and their drug resistance during 2009 to 2011 in Liangshan

Objective To understand the common clinical bacterial isolates and their drug resistance in Liangshan Prefecture.Methods Based on the National Clinical Tests′ Rules and Procedures,bacterial cultivation was carried out.Microscan PC20 and NC31 composite boards were used to identify the common bacteria and test their drug sensitivity,while K-B method was used to judge obligate aerobic bacteria.Statistics of related materials recorded during July 1st,2009 to June 30th,2011 were reviewed.Results 2 565 clinically suspected pathogenic strains were isolated,including 745 strains of Gram-positive bacteria,accounting for 29.1%,1 468 strains of Gram-negative bacteria,accounting for 57.2%,186 strains of Candida fungi,accounting for 7.3% and 166 strains of Genitourinary tract mycoplasma,accounting for 6.4%.The first five bacteria ranking in the amount of Gram-positive bacteria were Staphylococcus aureus,Staphylococcus epidermidis,Staphylococcus haemolyticus,Staphylococcus haemolyticus and Enterococcus faecalis,while the first five among Gram-negative bacteria were Escherichia coli,Acinetobacter baumannii,Pseudomonas aeruginosa,Klebsiella pneumoniae and Enterobacter cloacae.The sensitivity rate of Acinetobacter baumannii to Imipenem decreased to 35.5%.The sensitivity rate of Pseudomonas aeruginosa bacteria to Amikacin decreased to 42.0% and to Piperacillin decreased to 45.4%.The isolation rate of Acinetobacter baumannii with extensive drug resistance was 64.5%,of methicillin resistant staphylococcus aureus was 62.0%,of methicillin resistant coagulase negative staphylococcus was 90.5%,of Escherichia coli with extend-spectrum β-lactamases(ESBLs) was 77.9% and of Klebsiella pneumonia with ESBLs was 75.5%.Conclusion The situation of the bacterial drug resistance of the common clinical isolates might be very serious.It might be urgent to use antibacterial drugs properly and to strengthen infection control.