Study of Her2 expressing detection using FISH methods in breast cancer

Objective To study the detection of Her2 expression in breast cancer by using immunohistochemistry and fluorescence in situ hybridization(FISH) methods,and to evaluate their clinical value.Methods The expression of Her2 protein and gene in 51 cases with breast cancer were detected by immunohistochemical staining and FISH,respectively,and then the data was analyzed.Results Of 51 cases,the expression of Her2 protein were strongly positive in 15 cases(29.41%),positive in 13 cases(25.49%),weakly positive in 14 cases(27.45%),negative in 9 cases(17.65%);Her2 gene amplification existed in 19 cases(37.25%).No significant difference was found in the results between the two methods(χ2=3.76,P0.05),but the correlation coefficient of the two methods was not very well(r=0.539,P0.01),in the(+++),(++),(+) and(-) of Her2 protein expression,the Her2 gene amplication' was 80%,23.08%,28.57% and 0,respectively.Conclusion The immunohistochemistry is regarded as the initial screening method of the detection for Her2 expression.In the cases with positive expression of Her2 protein by immunohistochemistry,it must be further detected by FISH when it selects Herceptin in clinical therapy.