The comparative study between fluorescence in situ hybridization and immunohistochemistry in detection of breast cancer HER-2

Objective:To investigate the correlation between the expression of Her-2 gene observed using the fluorescence in situ hybridization(FISH) and the expression of Her-2 protein observed using immunohistochemistry(IHC) in patients of breast cancer.Methods 203 cases of breast cancer were detected by IHC technique and FISH technique respectively.Results In 203 cases of breast cancer,HER-2 was expressed in 54%(110/203) cases,while HER-2 was expressed in 29%(60/203) cases.In the 93 cases that the expression of HER-2protein was negative,there are 7 cases that the expression Her-2 gene was negative.In The 58 patients whose expression of HER-2 protein was positive,HER2 was expressed in 11 cases.In The 29 patients whose expression of Her-2 protein was middle positive,HER-2 was expressed in 22 cases.In the 23 patients whose expression of HER-2 protein was strong positive,HER-2 was expressed in 21 cases.Conclusion For the patients that were screened for the negative or strong positive,coincidence of the two methods is higher(≥ 91.3%),and FISH technique can be selected.In particular,more than 50-year-old age group of patients have a more coincidence(≥97.7%),while the patients with positive that were screened with IHC method should be carried out FISH experiments to determine the status of the HER-2 gene.