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2010•Journal of Chinese Practical Diagnosis and TherapyRequires access

Detection of HER-2 oncogene and the clinical significance in breast cancer

Liu Zhenggu

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Abstract

Objective To study the expressions of HER-2 in the protein and gene level in breast cancer and their clinical application.Methods HER-2 protein expression and gene amplification in 44 cases of breast cancer were detected with immunohistochemistry and fluorescence in situ hybridization respectively.Results Of these 44 cases,the expression of HER-2 protein was 3+ in 11 cases(25%),2+ in 19 cases(43%) and 1+ to 0 in 14 cases(32%).The amplification of HER-2 gene was positive in 10 cases(23%),and negative in 34 cases(77%).The coincidence of fluorescence in situ hybridization was 64%,16% and 100% respectively.The significant difference was found among three groups(P0.05).Conclusion The immunohistochemistry technique can be used in the initial screening of HER-2 gene overexpression in breast cancer.Only when it is combined with fluorescence in situ hybridization,it could be more accurate and objective for guiding clinical medication and judging the prognosis.

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What this paper is about

Objective To study the expressions of HER-2 in the protein and gene level in breast cancer and their clinical application.Methods HER-2 protein expression and gene amplification in 44 cases of breast cancer were detected with immunohistochemistry and fluorescence in situ hybridization respectively.Results Of these 44 cases,the expression of HER-2 protein was 3+ in 11 cases(25%),2+ in 19 cases(43%) and 1+ to 0 in 14 cases(32%).The amplification of HER-2 gene was positive in 10 cases(23%),and negative in 34 cases(77%).The coincidence of fluorescence in situ hybridization was 64%,16% and 100% respectively.The significant difference was found among three groups(P0.05).Conclusion The immunohistochemistry technique can be used in the initial screening of HER-2 gene overexpression in breast cancer.Only when it is combined with fluorescence in situ hybridization,it could be more accurate and objective for guiding clinical medication and judging the prognosis.

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Available abstract

Objective To study the expressions of HER-2 in the protein and gene level in breast cancer and their clinical application.Methods HER-2 protein expression and gene amplification in 44 cases of breast cancer were detected with immunohistochemistry and fluorescence in situ hybridization respectively.Results Of these 44 cases,the expression of HER-2 protein was 3+ in 11 cases(25%),2+ in 19 cases(43%) and 1+ to 0 in 14 cases(32%).The amplification of HER-2 gene was positive in 10 cases(23%),and negative in 34 cases(77%).The coincidence of fluorescence in situ hybridization was 64%,16% and 100% respectively.The significant difference was found among three groups(P0.05).Conclusion The immunohistochemistry technique can be used in the initial screening of HER-2 gene overexpression in breast cancer.Only when it is combined with fluorescence in situ hybridization,it could be more accurate and objective for guiding clinical medication and judging the prognosis.

Key concepts: Medicine, Immunohistochemistry, Breast cancer, Fluorescence in situ hybridization, In situ hybridization, Gene duplication, Clinical significance, Oncogene

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