Analysis of the distribution and antibiotic resistance of pathogenic bacteria isolated from blood samples of pediatric patients in Guangzhou

Objective To investigate the distribution and antibiotic resistance of pathogenic bacteria isolated from blood samples of pediatric patients and to provide essential information regarding the selection of antibiotic therapy for septicaemia.Methods All the blood samples isolated from patients in Guangzhou Children's Hospital between January 2005 and December 2006 were cultivated with Bactec9120 blood culture apparatus.Pathogenic bacteria were identified with API system and drug sensitivity tests were conducted with K-B method.Results Totally 385 strains of bacteria were isolated.208 strains of gram positive bacteria were isolated and accounted for 54%.Gram negative bacteria and fungi accounted for 42.6% and 3.4% respectively.Coagulase-negative staphylococcus was the dominant strains and comprised 35.8% of all isolates,followed by Klebsiella spp(8.8%),Acinetobacter(5.5%),Escherichia coli(4.9%),Pseudomonas aeruginosa(4.7%) and Staphylococcus aureus(4.4%).The main pathogenic bacteria in PICU was Acinetobacter and in NICU was Coagulase-negative staphylococcus,in hematopathy district was Enterobacteriaceae.The antibiotic resistance rate of Coagulase-negative staphylococcus against penicillin,ampicillin and erythromycin were more than 80%,but to vancomycin,teicoplanin and amikacin were lower.Enterobacteriaceae were susceptible to imipenem,amikacin and norfloxacin,whereas the resistance rate of them to ampicillin,piperacillin and third generation cephalosporins were high.Acinetobacter was resistant to most of monitored antibiotics,except for imipenem,cefoperazone/sulbactam.Conclusions Coagulase-negative staphylococcus was the most frequently isolated from blood samples of pediatric patients in Guangzhou.The distribution and antibiotic susceptibility of pathogenic bacteria should be considered when selecting antibiotic treatment in children with septicaemia.