Analysis of Volatiles from Irradiated Yeast Extract

The method for determination volatiles from irradiated yeast extract( YE) using headspace solid phase microextraction( HS-SPME) and gas chromatography-mass spectrometry( GC-MS) was developed in this paper. The extraction conditions were optimized with reference to the peak area and number of volatiles as aldehyde,ketone,alcohol,acid,ester and sulfur compounds. The optimized conditions of HS-SPME for volatiles in irradiated YE were: divinyl benzene / Carboxen / polydimethylsiloxane( DVB / CAR / PDMS) ber,extration time 40min,extraction temperature 40℃.The volatiles fromYE irradiated by 0- 19. 8kGy were detected using HS-SPME coupled with GC-MS. The results showed that only 15 volatiles were detected from no irradiated YE and main compounds were acetic acid,2,3- butanediol and 1- hexanol,2- ethyl-. There were 40 volatiles detected from irradiated YE and the main compounds were acetic acid,phenylethyl alcohol,heptanal and nonanal. Compare to no irradiated yeast extract,the aldehyde,ketone,alkene and disulfide,dimethyl were produced by irradiating process.