Analysis of Volatiles in Silver Carp by Headspace Solid Phase Microextraction Coupled with Gas Chromatography-Mass Spectrometry

The method for determination of volatiles using headspace solid phase microextraction(HS-SPME) and gas chromatography-mass spectrometry(GC-MS) was developed.The extraction conditions were optimized with reference to these volatiles as hexanal,heptanal,benzaldehyde,1-octen-3-ol,octanal,nonanal,decenal,2,4-heptadienal and 2,4-decadienal,and the optimized conditions of HS-SPME for volatiles in silver carp were as follows: divinyl benzene/carboxen/polydimethylsiloxane(DVB/CAR/PDMS) fiber,extration time(50 min),extraction temperature(60 ℃),the ratios of fish and saturated salt water(3∶7,m/V),desorption time(5 min).The volatiles in gills,viscera,scales and surimis of silver carp were detected using HS-SPME coupled with GC-MS.The results showed that the methods of HS-SPME were effective in extracting volatiles in silver carp.The types of volatiles in the gills were more than other tissues and the number of volatiles was 63,and the number of volatiles in viscera,scales and surimis was 48,44 and 42 respectively.These components of volatiles were mainly composed of saturated carbonyl compounds and alcohols,but unsaturated volatiles including aldehydes and enols contributed to the fishy off-odor.