Analysis of Volatile Compounds from Irradiated Yeast Extract by Headspace Solid Phase Micro-Extraction Coupled with Gas Chromatography-Mass Spectrometry

A method for determining volatile compounds from irradiated yeast extract using headspace solid phase microextraction(HS-SPME) and gas chromatography-mass spectrometry(GC-MS) was developed in this paper. The results showed that 13 volatile compounds were detected from unirradiated yeast extract including 1 acid, 2 aldehydes, 2 alcohols, 1 alkene and 3 nitrogen compounds and the major compounds were acetic acid, indole and 2-ethyl-1-hexanol. Totally 35 volatile compounds including 1 acid, 7 aldehydes, 2 alcohols, 4 alkenes, 2 ketones, 14 nitrogen compounds, 2 sulfur compounds, 1 ester and 2 benzenes were detected from irradiated yeast extract. The number and contents of volatile compounds increased with increasing irradiation dosage.