Effects of NF-Kappa B on the Expression of P-selectin in the Kidney of Diabetic Rats

Objective To investigate the expressions of P-selectin and nuclear factor-kappa B (NF-κB) in the kidney of diabetic rats,and their changes when the expression of nuclear factor -kappa B(NF-κB) was inhibited by PDTC.Methods 30 male Wistar rats were divided randomly into 3 groups:normal control group(NC group),diabetic model group(DM group) and diabetic rats treated with PDTC(a inhibitor of NF-κB)(DP group) with 10 rats in each group.Eight weeks later,blood glucose,glycosylated hemoglobin,kidney weight index and urine albumin excretion rate were determined.At the same time,the expressions of P-selectin and NF-κB were detected by immunohistochemistry.Results Expressions of P-selectin and NF-κB in the DM group were significantly higher than those in NC group(P0.01),the expressions of those in the DP group were significantly lower than those in the DM group,but significantly higher than those in NC group(P0.01).And the expression of p-selectin showed a strong positive correlation with that of NF-κB(r=0.954,P0.01).Conclusion In the renal tissue of diabetic rats,the expressions of NF-κB and P-selectin significantly increase, and inhibiting the expression of NF-κB can decrease the expression of P-selectin.