Effect of simvastatin on NF-kappa B p65 expression in liver of insulin resistant rats

Objective To study the effect of simvastatin(SV) on NF-kappa B p65 expression in liver of insulin resistant rats. Methods Male Wistar rats were divided into two groups:basic chow(NC,n=15)or high-fat diet(HF,n=20)for 10 weeks feeding, then assessed by euglycemic-hyperinsulinemia clamp technique. Rats in HF group were treated with(HFS,n=5)and without (HFN,n=5) SV 10mgkg-1d-1 in gavage for 5 weeks.NF-kappa B p65 expressions were tested with Western blot. Results The glucose infusion rate (GIR) in HF group decreased significantly compared with NC group (P0.01). The NF-kappa B p65 expression of liver in HFS group decreased significantly compared with HFN group (P0.05),but was not obviously different from NC group. Compared with NC group,insulin sensitivity index (ISI) was decreased significantly in HFS group and HFN group (P0.05). Conclusions SV may restore the NF-kappa B p65 expression in liver of rats with insulin resistance induced by high fat diet feeding,but could not improve systemic insulin resistance significantly.