Effect of NF-κB Inhibition on ICAM-1 of Diabetic Kidney

Objective:To investigate the effect of inhibition of NF-κB on ICAM-1 of diabetic kidney.Methods:30 healthy male Wistar rats were divided randomly into normal control group(group NC),diabetic model group(group DM)and diabetic rats with treatment of PDTC (group DP).Diabetic rats were induced by streptozotocin(STZ).At the end of 8th week,24 h urine was collected for UAER,and rats kidney were isolated to detect the expression of ICAM-1 by immunohistochemical staining.NRK cells were cultured in vitro and then divided into control groups with glucose in 5.6 mmol/L、15 mmol/L and 30 mmol/L,respectively;the experiment groups with PDTC in 5 μmol/L,10 μmol/L and 20 μmol/L on the basis of 30 mmol/L glucose.The expression of ICAM-1 mRNA was measured by RT-PCR after 24 h and 48 h,respectively.ICAM-1 was measured by ICC after 24 h and 48 h,respectively.Results:At the end of 8th week,the UAER in the group DM and DP were significantly higher than those in the group NC,and they decreased significantly in the group DP.The expression of ICAM-1 in the group DM were significantly higher than those in the group NC,and the expression of those in the group DP decreased significantly than those in the group DM,but still higher than those in the group NC.Compared with normal NRK cells groups,the ICAM-1 mRNA and protein were remarkably increased in a time-dose dependent manner exposed to different concentrations glucose (P0.05);the ICAM-1 mRNA and protein were remarkably decreased in a time-dose dependent manner exposed to different concentrations of PDTC (P0.05).Conclusion:Inhibition of NF-κB decreases the expression of ICAM-1 mRNA and protein in diabetic kidney,and that may play a salutary role in the occurrence and progression of diabetic nephropathy.