2006•Jilin Nongye Daxue xuebaoRequires access
Effects of Physical and Chemical Factors on Callus Growth and Flavonoid Production in Glycyrrhiza uralensis Calli
Shihai Yang
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Abstract
The effects of physical and chemical factors on callus growth and flavonoid production in Glycyrrhiza uralensis calli were studied.The results showed that fructose was better than sucrose for callus growth and flavonoid formation,and the optimum concentration was 2%.The flavonoids content was 2 times higher than that of sucrose as carbon sources.In the range of 2%～6% fructose concentration,the flavonoids content decreased with the increase of the concentration,but the licochalcone increased 5-fold.Light had no significant effect on callus growth,but enhanced the flavonoids production.The flavonoids content in light was 2 times higher than that of cultures in dark.The optimum temperature for callus growth and flavonoids production was 25℃.The callus growth and flavonoids formation were inhibited at 15℃ and 30℃.