2003Europe PMC (PubMed Central)Requires access

RAPD analysis of tea trees in Yunnan

Shao WanFang, Ruihua Pang, Pingsheng Wang, Xu Mei, Duan Hongxing, Zhang Ya-ping, Jiahua Li

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Abstract

RAPD assessment on genetic variations of 45 germplasm s in teas was carried out. 8 primers selected from 40 random primers were used to amplify 45 tea samples, and a total of 95 DNA bands were amplified, among which 90 (94.7%) were polymorphic. The average number of DNA bands amplified by each p rimer was 11.50. UPGMA cluster analysis of 95 DNA bands amplified by 8 primers w as made. The results showed that all the materials tested could be classified in to 7 groups including 5 complex groups and 2 simple groups, which was basically identical with morphological classification, besides these, there were some spec iation in 2 simple groups.

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What this paper is about

RAPD assessment on genetic variations of 45 germplasm s in teas was carried out. 8 primers selected from 40 random primers were used to amplify 45 tea samples, and a total of 95 DNA bands were amplified, among which 90 (94.7%) were polymorphic. The average number of DNA bands amplified by each p rimer was 11.50. UPGMA cluster analysis of 95 DNA bands amplified by 8 primers w as made. The results showed that all the materials tested could be classified in to 7 groups including 5 complex groups and 2 simple groups, which was basically identical with morphological classification, besides these, there were some spec iation in 2 simple groups.

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Available abstract

RAPD assessment on genetic variations of 45 germplasm s in teas was carried out. 8 primers selected from 40 random primers were used to amplify 45 tea samples, and a total of 95 DNA bands were amplified, among which 90 (94.7%) were polymorphic. The average number of DNA bands amplified by each p rimer was 11.50. UPGMA cluster analysis of 95 DNA bands amplified by 8 primers w as made. The results showed that all the materials tested could be classified in to 7 groups including 5 complex groups and 2 simple groups, which was basically identical with morphological classification, besides these, there were some spec iation in 2 simple groups.

Key concepts: RAPD, UPGMA, Germplasm, Biology, DNA, Genetics, Veterinary medicine, Genetic diversity

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