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2007•Acta Horticulturae SinicaRequires access

RAPD Analysis on the Genetic Relationships of Tea Cultivars Grown in Sichuan

Zhang Ze-cen

Open publisher page 2 citations

Abstract

The genetic relationship of 36 tea cultivars grown in Sichuan was studied by RAPD technique.Sixteen primers selected from 50 random primers were used to amplify 36 tea samples,and a total of 167 DNA bands were amplified,among which 158(94.61%)were polymorphic.Genetic distances between the cultivars varied from 0.149 to 0.679.UPGMA cluster analysis of 167 bands amplified by 16 primers was made.The result showed that 36 tea cultivars tested could be classified into 3 complex groups and 2 simple groups.It was found that there was a certain correlation between the results and the derivation of tea cultivars.

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The genetic relationship of 36 tea cultivars grown in Sichuan was studied by RAPD technique.Sixteen primers selected from 50 random primers were used to amplify 36 tea samples,and a total of 167 DNA bands were amplified,among which 158(94.61%)were polymorphic.Genetic distances between the cultivars varied from 0.149 to 0.679.UPGMA cluster analysis of 167 bands amplified by 16 primers was made.The result showed that 36 tea cultivars tested could be classified into 3 complex groups and 2 simple groups.It was found that there was a certain correlation between the results and the derivation of tea cultivars.

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Available abstract

The genetic relationship of 36 tea cultivars grown in Sichuan was studied by RAPD technique.Sixteen primers selected from 50 random primers were used to amplify 36 tea samples,and a total of 167 DNA bands were amplified,among which 158(94.61%)were polymorphic.Genetic distances between the cultivars varied from 0.149 to 0.679.UPGMA cluster analysis of 167 bands amplified by 16 primers was made.The result showed that 36 tea cultivars tested could be classified into 3 complex groups and 2 simple groups.It was found that there was a certain correlation between the results and the derivation of tea cultivars.

Key concepts: RAPD, Cultivar, UPGMA, Genetic relationship, Biology, Horticulture, Botany, Veterinary medicine

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