Study on the Genetic Diversity of Peculiar Tea Germplasm Resource in Yunnan by RAPD
Duan Hongxing
Abstract
Duan Hongxing
Abstract
Random Amplified Polymorphic DNA (RAPD) technique was used to analysis the genetic diversity of 48 materials,Which including wild type old tea tree,intermediate type old tea tree,cultivated type species,wild species,local variety and the kidney plant:Camellia fascicularis. The classification and blood relationship among 48 germplasm were explored from DNA level. No single-morphic band has been found among the total 112 DNA bands. The genetic diversity degree was 100%. The figure showed that the tea trees in Yunnan processing much high genetic diversity on DNA molecular level. The genetic distance was between 0.116 to 0.527 and the average was 0.202. All the RAPD amplified bands were clustered by Unweighted Pair Group with Mathematic Average (UPGMA) based on Euclidean distances. The dendrogram of UPGMA showed that the 48 materials could be classified into 5 groups including 3 complex groups and 2 simple groups, which was basically identical with morphological classification.
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Random Amplified Polymorphic DNA (RAPD) technique was used to analysis the genetic diversity of 48 materials,Which including wild type old tea tree,intermediate type old tea tree,cultivated type species,wild species,local variety and the kidney plant:Camellia fascicularis. The classification and blood relationship among 48 germplasm were explored from DNA level. No single-morphic band has been found among the total 112 DNA bands. The genetic diversity degree was 100%. The figure showed that the tea trees in Yunnan processing much high genetic diversity on DNA molecular level. The genetic distance was between 0.116 to 0.527 and the average was 0.202. All the RAPD amplified bands were clustered by Unweighted Pair Group with Mathematic Average (UPGMA) based on Euclidean distances. The dendrogram of UPGMA showed that the 48 materials could be classified into 5 groups including 3 complex groups and 2 simple groups, which was basically identical with morphological classification.
Key concepts: UPGMA, RAPD, Germplasm, Genetic diversity, Dendrogram, Biology, Genetic distance, Botany