Detection and Significance of β-Glucuronidase mRNA in Human Liver and Kidney Tissues

Objective To establish a new method for detection of β-glucuronidase(β-G) mRNA in human liver and kidney tissues.Methods β-G mRNA expression was detected by reverse transcription polymerase chain reaction(RT-PCR) in 10 cases of normal liver tissues,10 cases of normal kidney tissues and 8 cases of hepatocellular carcinoma tissues.Results The expand products of β-G mRNA were expressed in liver and kidney tissues with similar size of 422 bp.The expression contents of β-G mRNA in liver and kidney normal tissues were different(1.71±0.32 vs 1.83±0.22) but without statistical significance(P0.05).However,the expression content of β-G mRNA in hepatocellular carcinoma tissues was 3.88±0.86,which was significantly higher(P0.01) than that in normal liver tissues.Conclusion β-G mRNA determination is feasible by checking β-G gene alignment in gene bank and designing draw matter in the tissue of liver and kidney.It may be very significant to explore the change of β-G mRNA in various tissues in studying of molecular mechanism.