2003•PubMedRequires access

[Expressions of angiotensin II type 1 receptor mRNA in fibrotic and normal liver tissues].

Weiwei Wang, Xi-shan Yang, Ping‐sheng Wu, Jie Wang, Hongbin Zhang, Xu Li, Wenling Zheng

Abstract

OBJECTIVE: To investigate the expression profile of angiotensin type 1 receptor (AT1R) mRNA in fibrotic and normal liver tissues. METHODS: Semi-quantitative RT-PCR was used to detect the expression of AT1R mRNA in 18 specimens of fibrotic liver tissues adjacent to liver cancers and 12 normal liver tissue specimens. Specific AT1R product and GAPDH product used as internal control were both amplified by RT-PCR, and the ratio of their integrated optical densities calculated to estimate the relative quantity of AT1R mRNA expression. RESULTS: The relative mRNA expression of AT1R in fibrotic liver tissues was significantly higher than that of normal liver tissues (1.27+/-0.45 vs 0.71+/-0.21, P < 0.01). CONCLUSION: AT1R may play an important role in the development of hepatic fibrosis.

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Key concepts: Messenger RNA, Angiotensin II, Glyceraldehyde 3-phosphate dehydrogenase, Real-time polymerase chain reaction, Receptor, Biology, Pathology, Endocrinology