Optimization of Purification Technology for Total Flavonoids from Callicarpa nudiflora by Polyamide Resin

Objective: To optimize purification technology of total flavonoids from Callicarpa nudiflora by polyamide resin. Method: With the content of total flavonoids as index, adsorption and elution behavior of polyamide resin for total flavonoids was investigated by stastic-dynamic adsorption-elution test,single factor tests were adopted to optimize purification technology for total flavonoids taking saturated adsorption, pH, elution volume and other parameters. Result: Optimum purification technology parameters were as follows: the concentration of sample solution 0. 073 g·mL- 1,sample volume of 2 BV,pH of sample solution 3. 0,eluted impurities with 3 BV water,then eluted with 3 BV of 80% ethanol; Purity of total flavonoids was up to 42. 3% from 5. 6%, average retention rate was 89. 6%( RSD 3. 56%). Conclusion: Purification effect of total flavonoids from C. nudiflora by polyamide resin was feasible,and this study could provide experimental basis for development of new formulations from C. nudiflora.