AN INVESTIGATION IN THE ANTITUMOR EFFECT OF SODIUM DIMERCAPTOPROPANSULFONATE BOTH IN VIVO AND IN VITRO

Objective To investigate the antitumor effect of two thiols-bearing compound-sodium dimercaptopropansulfonate(DMPS)both in vivo and in vitro.Methods The human pulmonary adenocarcinoma cell line A549 cultured in vitro was used to test the effect of DMPS with different concentrations on the growth and proliferation of the tumor cells by means of MTT method,and to test the apoptosis induction of DMPS with different concentrations via fluorescence microscope and with cell apoptosis-Hoechst33258 kit.The inhibitory effect of DMPS with different concentrations on the tumor growth in the model of mice sarcomas 180 was investigated.Results In vitro,DMPS,at the final concentrations of both 8mmol/L and 16mmol/L,significantly inhibited the tumor cell growth and its proliferation when administered 48h(P0.01) and 72h(P0.01) later respectively.The final concentrations of both 8mmol/L and 16mmol/L of DMPS significantly increased the number of tumor cells with apoptotic signs when administered 6h(P0.05) and 9h(P0.01) later respectively.In vivo,1.5%DMPS was administered daily intraperitoneally at a dose of 0.2ml/10 weight body for 2 weeks significantly reduced the tumor mass(P0.05).Conclusion High concentrations of DMPS can significantly inhibit the growth and proliferation of both A549 cell line in vitro and s180 cell line in vivo.