Antitumor effects of Combretaftation A-4P in vivo and in vitro

OBJECTIVE To investigate the in vivo/in vitro inhibitory effects of compound Combretaftation A-4P(CA-4P) on human leukemia HL-60 cell line and on HL-60 cell inoculated with transplant tumor nude mice.METHODS The inhibitory effect of compound CA-4P on human leukemia cell line HL-60 was assayed by MTT analysis,cell growth curve and clone forming experiments with different concentrations of CA-4P.The change of cell configuration was observed by dyeing.The inhibitory effect of compound CA-4P on nude mice inoculated with HL-60 cells in vivo was demonstrated by the prolongation of survival times in mice carried tumor.RESULTS After treated with CA-4P for 48 hours,MTT assay showed the dose range from 0.5 μg·ml-1 to 64.0 μg·ml-1 had the inhibitory ratio range from 15% to 79%.The average IC50 in 48 h was 6.579 μg·ml-1.The dose-effect relationship was described in the growth curve.The characteristic typical morphology for apoptosis was observed under microscope.The dose-effect relationship was also observed in the experimentation of HL-60 cell inoculated transplant tumor nude mice by CA-4P, and the prolongation of survival times in mice carried tumor was prolonged along with the rise of CA-4P concentration.CONCLUSION CA-4P could inhibit the growth of HL-60 cell in vitro,and its in vivo inhibitory effectin of HL-60 cell inoculated nude mice was distinct.