2006•Zhongguo yaofangRequires access

Determination of Ginsenoside Rg_1 in Naoling Oral Liquid by HPLC

Gong Jingmiao

Abstract

OBJECTIVE:To establish an HPLC method for the determination of Ginsenoside Rg1 in Naoling oral liquid.METHODS:The assaying was conducted on a Hypersil BDS-C18 with methol-water(49∶ 51)as mobile phase.The detective wavelength was set at 203nm;the column temperature was 30℃ ;the flow rate was 1.0ml/min and the sample size was 20μ l.RE_SULTS:The linear concentration range of Ginsenoside Rg1 was 0.2μ g～ 8.0μ g(r=0.9 996);the average recovery was 101.25%(RSD=1.54%).CONCLUSION:The method is simple,accurate and reproducible;and it can be used for the quality control of Ginsenoside Rg1.

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Key concepts: Ginsenoside Rg1, Chromatography, High-performance liquid chromatography, Ginsenoside, Chemistry, Linear range, Linear relationship, Content determination