2012•Biotechnology(Faisalabad)Requires access

Phylogenetic Analysis of 16S rDNA Sequence and PCR - RFLP of Bacillus from Fumao - flavor Daqu

Yan Lin-chun, Shou-Cai Zhang, Xiaowei Ma, Tang Er-jiang, Huang Zu-xin, Youqiang Chen

Abstract

Objective:89 strains of Bacillus were isolated from Daqu of a Sauce-flavor distillery in Fujian,finally we preliminarily identified and obtained 18 Bacilluses for microbial diversity research.Method:The 16S rDNA of 18 Bacilluses were researched with PCR-RFLP and System growth analysis.Result:They were grouped into four groups by 16S rDNA PCR-RFLP,and then categorized into eight kinds(6 Bacillus subtilis,4 Bacillus cereus,2 Bacillus licheniformis,2 Bacillus sonorensis,1 Bacillus pumilus,1 Bacillus oleronius,1 Bacillus thuringiensis,1 Bacillus coagulan)by 16S rDNA sequence comparison.Conclusion:The cultured Bacilluses from the high temperature Daqu has the microbial diversity

About this research paper

What this paper is about

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Bacillus pumilus, Bacillus licheniformis, 16S ribosomal RNA, Biology, Bacillus (shape), Bacillus subtilis, Bacillus cereus, Microbiology