Systematic Analysis of Complete Sequence of 16S rDNA of Bacillus in the Fermented Grain

69 strains of Bacillus were isolated from the fermented grains in the pits for Luzhou-flavor liquor, then they were divided into 5 groups according to their morphological and physiological characteristics. The complete sequence of 16S rRNA of the representative strains for the 5 groups were amplified by PCR, and then sequenced. Homology analysis was made by searching in Genbank with NCBI-Blast, and the phylogenetic tree was reconstructed. The results indicated that bacillus were mainly bacillus licheniformi and bacillus amyloliquefaciens. In addition, bacillus subtilis and bacillus circulans also had high percentage, and some amount of bacillus cereus, which proved the high biodiversity of bacillus in fermented grains and there was no obvious dominated strain there.