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The TD-PCR and Phylogenetic Analysis of Bacillus licheniformis 16S rDNA

Guang Liu

Abstract

16S rDNA sequences of 30 Bacillus strains originally identified as Bacillus licheniformis from China Center of Industrial Culture Collection (CICC) were determined and analyzed. The results indicated that 24 strains are affiliated to Bacillus licheniformis;3 strains are affiliated to Bacillus cereus and 1 strain is affiliated to Bacillus subitilis;the similarity levels of 16S rDNA among the rest of 2 strains and other strains of Bacillus licheniformis,range from 96.4% to 97.4%,further tests are needed to clarify their position. Also we testified that 5' terminal 500bp of 16S rDNA is available to differentiate the strains of Bacillus licheniformis、Bacillus subtilis and Bacillus cereus.

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Key concepts: Bacillus licheniformis, Bacillus cereus, 16S ribosomal RNA, Bacillus (shape), Bacillus subtilis, Biology, Microbiology, Bacillales