2006•Jingji dongwu xuebaoRequires access
Cloning and Comparing H Gene of Vaccine Strain of Canine Distemper Virus
Quankai Wang
Open publisher page 0 citations
Abstract
One pair of primers was designed and synthesized based on the haemagglutinin(H) protein gene sequence of the Onderstepoort strain of canine distemper virus(CDV) The total RNA was isolated from the Vero cells line infected with the vaccine strain of CDV.The products of reverse transcription(RT) reaction were used as PCR templates.The H gene fragment was amplified by polymerase chain reaction(PCR).A 843 bp nucleotide fragment of the PCR products was connected with the vector pMD18-T.The recombinant plasmid was identified by restriction endonuclease KpnⅠ/ApaⅠand PCR.and then sequenced.The results showed that the H gene sequence of the vaccine strain shared homology of 95.9%.with that of the Onderstepoort CDV strain