2006•Anhui Medical and Pharmaceutical JournalRequires access

Determination of astragaloside IV in astragalus by HPLC-ELSD

Ju-Tao Wang, XU Wen-fang, Jinqi Liu, MA Feng-yu

Abstract

AimTo determine astragaloside Ⅳ in Astragalus by HPLC-ELSD.MethodsHPLC-ELSD was used in the quantitative analysis of astragaloside Ⅳ in the sample.The separation was performed on Hanbang C_(18)(4.6 mm×150 mm,5 μm)column with a mixture of acetonitrile-water(40:60) as mobile phase.The flow rate was 1.0 ml·min~(-1).The temperature of drift tube for measurement was 105℃,and the flow rate of N_(2) was 2.8 L·min~(-1).ResultsThe standard curve was linear in the range of 4.0～18.0 μg for astragaloside Ⅳ(r=0.9999,n=6).The average recovery was 98%.ConclusionThe method is simple,reliable,and is a good method to determine astragaloside Ⅳ.

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Key concepts: Astragaloside, Drift tube, Chromatography detector, Chromatography, High-performance liquid chromatography, Astragalus, Chemistry, Linear range