Effects of Matrine on proliferation in vitro and on apoptosis of PA-1 human ovary malignant teratoma cell line

Objective:To study the effects of matrine on proliferation in vitro and apoptosis of PA-1 human ovary malignant teratoma cell line. Methods: The inhibition ability of matrine on the cell line was detected with MTT assay and the dose-effect relationship and the time-effect relationship were found. The morphological changes of the cells with DAPI dyeing was observed. Apoptosis rates and cell cycle were detected with flow cytometry. Results: The inhibition rates of PA-1 cells were decreased according to matrine's concentration and time. While observed by DAPI dyeing, there was obviously karyopycnosis in the cells. The result of flow cytometry showed that matrine could induce apoptosis of PA-1 cells, stop the growth of them at G1 period and restrain mitosis of cells.And the apoptosis rates of 1.0,0.5,0.25 mg/ml concentration of matrine were declined in order after they affected the cells for 24 hours. Conclusion: Matrine can inhibit the growth of PA-1 cells through inducing apoptosis. The apoptosis effect is dose-dependent.