DEPRESSANT EFFECT OF SILYBIN-PHOSPHATIDYLCHOLINE COMPOUND(SPC)ON ADVANCED GLYCATION END PRODUCTS (AGEs) IN ALLOXAN-INDUCED DIABETIC MICE

[Objective]To investigate the depressant effect of silybin-phosphatidylcholine compound(SPC)on the formation of advanced glycation end products(AGEs)in alloxan-induced diabetic mice.[Methods]Among seventy mice,ten mice were randomly selected as normal controls,the others were injected with 80 mg·kg-1 alloxan through vena caudalis respectively.The level of blood-fasting sugar of the mice were measured after 72 hours,and 41 diabetic mice whose blood glucose was higher than 16.7 mmol·L-1 were randomly divided into four groups:model group,high dose of SPC group,medium dose of SPC group and low dose of SPC group.Mice in normal control group and model group were administered with 0.5% CMC-Na suspension for 12 weeks,while mice in three SPC groups were respectively administered with 400 mg·kg-1,200 mg·kg-1 and 100 mg·kg-1 SPC mixed with 0.5% CMC-Na suspension for 12 weeks.The blood glucose level of vena caudalis were detected at the fourth,eighth and twelfth week,and at the end of the twelfth week,the serum levels of creatinine(Crea),urea nitrogen(BUN)and fructosamine(Fmn)were detected,the levels of MDA and SOD in nephridial tissue and the level of AGEs in cortex renis were detected.[Results]SPC had no significant effect on the blood glucose level of diabetic mice(P﹤0.05),but could obviously decrease the serum levels of Crea,BUN and Fmn,as well as the content of AGEs in cortex renis;besides,SPC had obvious depressant effect on the decrease of SOD activity and the increase of MDA value in nephridial tissue compared with model group(P﹤0.05).[Conclusions]High and medium dose of SPC can inhibit the formation of advanced glycation end products in alloxan-induced diabetic mice.