Protective effect of soybean isoflavone on morphology of vein endothelial cells from oxidative injury in vitro

Objective:To observe the protective effect of soybean isoflavone(SI)on the morˉphology of vein endothelial cells(ECV)which were damaged by oxidized low-density lipoproˉtein(ox-LDL)in vitro.Methods:Vein endothelial cells were cultured in vitro and divided into5groups which were ox-LDL,VE+ox-LDL,(SI-L?M?H)+ox-LDL groups,respectiveˉly.Endothelial cells were incubated with ox-LDL for24hours after incubated with vitamin E(VE)and different dose of SI for24hours.The level of MTT(OD)was measured and general morphology and ultrastructure of ECV was observed.Results:The value of MTT(OD)in ox-LDL group were remarkable lower than that in the other groups(P0.05).Between VE+ox-LDL group and(SI-L?M?H)+ox-LDL groups,there was no significant difference(P0.05).The observation of optical microscope,ECV was contractive and circinal in ox-LDL group.On the contrary,ECV was polygon or short shuttle state in the other groups.Beˉsides,the ultrastructure of ECV in VE+ox-LDL group or(SI-L?M?H)+ox-LDL groups was better than that of ox-LDL group.Conclusion:SI has definite protective effects on human vein endothelial cells from ox-LDL induced injury on morphology.