2007•Journal of Yangtze University MedicineRequires access
Effect of Tetramethylprazine on Oxidative Low-Density Induced Endothelial Cell Injury
Yun Wei
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Abstract
Objective:To observe the effect of Tetramethylprazine (Tet) on endothelial cell(ECV304) injured by oxidative low-density lipoprotein (ox-LDL).Methods:Cultured ECV304 cells were divided into 4 groups: Control group (ECV304),Tet control group (ECV304+ Tetramethylprazine),ox-LDL group (ECV304+ox-LDL) and Tet group (ECV304+ox-LDL+Tetramethylprazine).The concentrate of ox-LDL and Tet used in the experiment was 0.1 mg/ml and 0.5 mg/ml respectively.After dealing with different treatments,cells of four groups cultured for further 24 hours,and then culture medium was collected for the measurement of LDL,MDA contents.The activities of SOD and GSH-PX were detected in ECV304 cells.Result:The contents of LDH and MDA in Tet group were significantly lower than ox-LDL group (P0.05);The activities of SOD and GSH-PX in Tet group were significantly increased than ox-LDL group (P0.05).Conclusion: Tetramethylprazine has the protective function of the endothelial cells injury induced by ox-LDL via a anti-oxidation effect.