RAPD Markers Linked to the Powdery Mildew Resistance Gene in Wheat Line Gui-nong 775

Random Amplified Polymorphic DNA (RAPD) was employed to detect molecular markers linked to powdery mildew resistance gene in wheat line Gui-nong 775. One DNA fragment was amplified in the resistant parent Gui-nong 775 and resistant materials by a primer S2018, but not in the susceptible materials and susceptible parent Feng-chan 3. The DNA fragment length was about 880 bp. The genetic linkage of the DNA fragment with powdery mildew resistance gene was tested on a segregating F 2 population (106 plants). The result showed that the DNA fragment was closely linked to the powdery mildew resistance gene in wheat line Gui-nong 775, in which the genetic distance between S2018 880. and powdery mildew resistance gene was 8.9 cM. The linkage molecular markers were approved to be credible by other relative progeny materials.