GENETIC ANALYSIS AND RAPD MARKERS OF POWDERY MILDEW RESISTANCE GENE IN WHEAT LINE 2-26
Tihua Fu
Abstract
Tihua Fu
Abstract
Powdery mildew,caused by Blumeria Graminis f.sp.tritici,is one of the most important damaging diseases in many regions of the world.In present study,genetic analysis of resistance for powdery mildew in a stable wheat line 2-26,which was derived from the hybrid progeny between Triticum durum-Dasypyrum villosum amphiploid and common wheat,was carried out.The result indicated the powdery mildew resistance in line 2-26 is controlled by a single dominant gene and temporarily named Pm2-26.Molecular markers and bulked sergeant analysis were used to characterized the powdery mildew resistance gene.Two RAPD markers(SBSC2 and SBSI20) were found tightly linked to the resistance gene in line 2-26.These results provided a basis to further transfer into stable SCAR marker in future.In addition,the gene origin in line 2-26 and relationships with other resistance genes were also discussed in this paper.
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Powdery mildew,caused by Blumeria Graminis f.sp.tritici,is one of the most important damaging diseases in many regions of the world.In present study,genetic analysis of resistance for powdery mildew in a stable wheat line 2-26,which was derived from the hybrid progeny between Triticum durum-Dasypyrum villosum amphiploid and common wheat,was carried out.The result indicated the powdery mildew resistance in line 2-26 is controlled by a single dominant gene and temporarily named Pm2-26.Molecular markers and bulked sergeant analysis were used to characterized the powdery mildew resistance gene.Two RAPD markers(SBSC2 and SBSI20) were found tightly linked to the resistance gene in line 2-26.These results provided a basis to further transfer into stable SCAR marker in future.In addition,the gene origin in line 2-26 and relationships with other resistance genes were also discussed in this paper.
Key concepts: Powdery mildew, Blumeria graminis, Biology, Common wheat, RAPD, Genetic analysis, Genetics, Gene