RAPD markers linked to the powdery mildew resistance gene in wheat line Guinong 001

Random Amplified Polymorphic DNA (RAPD) and BSA method were employed to detect molecular markers linked to powdery mildew resistance gene in wheat line Guinong 001. The results showed the DNA fragments were amplified in the resistant parent Guinong 001 and resistant materials by one primer S2018, but the fragments were not amplified in the susceptible materials and susceptible parent long 96-6239. The DNA fragment amplified by the primer S2018 was about 900bp. The genetic linkage of the DNA fragment by the primer S2018 with powdery mildew resistance gene was tested on a Segregating F_2 population (110 plants).The result showed that the DNA fragment was closely linked to the powdery mildew resistance gene in wheat line Guinong 001. In which the genetic distance between S2018 and powdery mildew resistance gene was 1.7cM. The markers acquired would offer an effective elected means to transfer the powdery mildew resistance gene in wheat Guinong 001 to other wheat materials.