Study on the Extraction Technology of Polysaccharide from Allium macrosttemon B.

The extraction of polysaccharide from Allium macrosttemon B.was studied.With single factor test and orthogonal experiment design method we analyzed the influence of factors such as temperature(A),solid-liquid ratio(B) and time(C) on the extraction percent of the polysaccharides.The results indicated that the influence of the three factors on extraction percent of the polysaccharides was ACB and the temperature was the main factor.The optimum extraction technology was A_3B_2C_3(temperature was 90?℃,solid-liquid ratio was 1∶12 and the extraction time was 90 min).Under these conditions,the extraction percent of soluble polysaccharides from Alluim macrosttemon B.was 13.93%.In addition,the method of removing protein was studied and the results indicated that Sevag method combined with the enzyme was best.