Study on differentially expressed genes in myocarditis by cDNA microarray

Objective To search for the differentially expressed genes in myocarditis tissue and normal tissue using cDNA microarray. Methods The PCR products of 8 464 genes were spotted on a chemical-material-coated-glass plate in array. DNAs were fixed onto the glass plate after series of treatments. The total RNAs were isolate from the tissues, and were purified to mRNAs by Oligotex. Both mRNAs from the myocarditis tissue and normal issue were reversely transcribed to the cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The mixed probes were hybridized to the cDNA microarray. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between 2 tissues. Results Among the 8 464 target genes, 1 684, 284, 98 genes differentially expressed in 3 samples were identified. The analysis showed that there were significant correlations between PIM-3, angiopoietin-1, Histocompatibility2 and myocarditis. Conclusions cDNA microarray technique is effective in screening the differentially expressed genes between myocarditis tissue and normal tissue. The downregulation of PIM-3, angiopoietin-1, Histocompatibility2 may be responsible for the pathogenesis and progression of viral myocarditis.