2013•Research of Integrated Traditional Chinese and Western MedicineRequires access
Effects of Intensifying Kidney and Spleen and Activating Blood Circulation Decoction on the Express of IL-17 and OPG/RANKL in Serum of CIA Rat
YU Jianpin
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Abstract
Objective To explore the effects of intensifying kidney and spleen and activating blood circulation decoction on the express of IL-17 and OPG/RANKL in serum of CIA rat.Methods The CIA rat model was induced by intradermal of CFA and typeⅡcollagen.A total of 60 Wistar rats were divided into 5 groups randomly,three treatment groups which were given high,medium and low concentration of intensifying kidney and spleen and activating blood circulation decoction respectively,control group which was given normal saline and CIA mode group.After being lavaged for 30 days,the level of IL-17,OPG and RANKL in serum was determined by ELISA kits.Results Compared with the model group,the level of IL-17 and RANKL in rat serum of three treatment groups decreased significantly(P0.05),whereas the serum level of OPG in three treatment groups increased markedly(P0.05).Conclusion The intensifying kidney and spleen and activating blood circulation decoction could postpone joint bone inflammatory damage through reducing the serum levels of IL-17 and RANKL and increasing the serum level of OPG in the of CIA model rat.