Generation and Characterization of Monoclonal Antibodies Against Canine Distemper Virus

Canine distemper virus was purified and used to immunize Balb/c mice.The hybridoma culture supernants were screened by indirect ELISA.The positive cells were subcloned until all clones were positive.The specificity of monoclonal antibodies was confirmed by Vero cross-reaction test and IFA.The results showed that three hybridoma cells secreting anti-CDV McAb were obtained.These two cells were named062-1,062-2.The lab had one McAb called 6H8.All the three McAbs confirmed by cross-reaction test and IFA were specific against CDV.The positive hybridomas were also able to secrete McAbs stably by series passage in vitro with freezing and recovering.The titers of cell culture supernant and ascites tested by ELISA were 1∶105 and 1∶108,respectively.Additivity ELISA results showed that epitopes recognized by2McAbs(062-1,062-2)differed from that of by McAb 6H8.