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2008•Qilu Pharmaceutical AffairsRequires access

Determination of β-carotene in Damaicao Tablets by HPLC

XU Zhen-qiang

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Abstract

OBJECTIVE To establish a method for determination of β-carotene in Damaicao Tablets by reversed-phase high performance liquid chromatography.METHODS Chromatographic conditions:using Capcell Pak UG120 C18(4.6mm×150mm,5um)as chromatographic column,methanol-acetonitrile(90∶10)as mobile phase;The flow rate was 2mL·min-1,detection wavelength was 450nm.RESULTS The method was proved to be linear in the ranger of 7~105μg·mL-1(r=0.9994),RSD was 1.27 %,the average recovery was 99.34%.CONCLUSION The method was simple,rapid,accurate,sensitive and reproducible.It was suitable for determination of β-carotene in Damaicao Tablets.

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What this paper is about

OBJECTIVE To establish a method for determination of β-carotene in Damaicao Tablets by reversed-phase high performance liquid chromatography.METHODS Chromatographic conditions:using Capcell Pak UG120 C18(4.6mm×150mm,5um)as chromatographic column,methanol-acetonitrile(90∶10)as mobile phase;The flow rate was 2mL·min-1,detection wavelength was 450nm.RESULTS The method was proved to be linear in the ranger of 7~105μg·mL-1(r=0.9994),RSD was 1.27 %,the average recovery was 99.34%.CONCLUSION The method was simple,rapid,accurate,sensitive and reproducible.It was suitable for determination of β-carotene in Damaicao Tablets.

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Available abstract

OBJECTIVE To establish a method for determination of β-carotene in Damaicao Tablets by reversed-phase high performance liquid chromatography.METHODS Chromatographic conditions:using Capcell Pak UG120 C18(4.6mm×150mm,5um)as chromatographic column,methanol-acetonitrile(90∶10)as mobile phase;The flow rate was 2mL·min-1,detection wavelength was 450nm.RESULTS The method was proved to be linear in the ranger of 7~105μg·mL-1(r=0.9994),RSD was 1.27 %,the average recovery was 99.34%.CONCLUSION The method was simple,rapid,accurate,sensitive and reproducible.It was suitable for determination of β-carotene in Damaicao Tablets.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Acetonitrile, Carotene, Methanol, Detection limit

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