Investigation of biodegrading characteristics of BTEX by low-temperature-tolerated bacterial colony

The article taked the nitrobenzene and BTEX-polluted soil sample fron Jihua double benzene factory as the bacteria source,obtaining the low temperature and highly effective BTEX degrading bacteria colony by the screening and domestication.The indoor research indicated that on the condition of 10 ℃,PH=6.8,BTEX total mass concentration 200 mg·L-1,the individual removal of B,T,E,X was 93.2%、95.6%、91%、88.4% in 84 h.As we know,Benzene was the most difficult one to be degraded among the 6 kinds of BTEX.So we taked benzene to simulate degrading kinetics curve,When the initial mass concentration of benzene was less than 17.48 mg·L-1,degradation conformed to first order degrading kinetics,When the initial mass concentration of benzene varied between 17.48-195.30 mg·L-1,gradation conformed to zero-order degrading kinetics.The bacteria colony was very stable and adaptable,after the transfer of 80 generations,the bacteria colony's degrading abilities was as well as before.And the degrading abilities of the bacteria colony in 6 different ingredient ground water was still high.Biolog system and 16Sr DNA gene analysis revealed that B2 is Stenotrophomonas maltophilia,B4 is Pseudomonas maculicola,B6 is Pseudomonas spinosa.