2013•Zhongguo shengwu gongcheng zazhiRequires access

Isolation of a Fungal Strain of Degrading BTEX and Its Degradation Characteristics

Zhansheng Zhang, Chunhu Li, DU Qing-ping, Xia Li-juan

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Abstract

By gradually increasing the concentration of BTEX in the culture medium with toluene,benzene and xylene as the sole carbon source,a BTEX-degrading fungi strain which was designated as B1 was isolated from the sludge of sewage treatment plant.Using single factor and orthogonal experiment analyzed and studied the fungal degradation environment effect factors and degradation efficiency.The results showed that the optimum condition of fungal degradation was C∶N = 5∶1,pH5,temperature 30 ℃,strains inoculation amount 5.5ml(50ml culture medium).GC analysis was used to determine the fungal degradation effect to BTEX with the initial liquid phase concentration of 0~90mg/L,the results showed that the degradation activities of fungal strain was not inhibited,and the fungal degradation efficiency was toluene benzene xylene,the highest degradation efficiency reached 87.39%,85.21%,81.47%,respectively.The degradation efficiency of mixed substrates was slightly higher than single substrate.

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What this paper is about

By gradually increasing the concentration of BTEX in the culture medium with toluene,benzene and xylene as the sole carbon source,a BTEX-degrading fungi strain which was designated as B1 was isolated from the sludge of sewage treatment plant.Using single factor and orthogonal experiment analyzed and studied the fungal degradation environment effect factors and degradation efficiency.The results showed that the optimum condition of fungal degradation was C∶N = 5∶1,pH5,temperature 30 ℃,strains inoculation amount 5.5ml(50ml culture medium).GC analysis was used to determine the fungal degradation effect to BTEX with the initial liquid phase concentration of 0~90mg/L,the results showed that the degradation activities of fungal strain was not inhibited,and the fungal degradation efficiency was toluene benzene xylene,the highest degradation efficiency reached 87.39%,85.21%,81.47%,respectively.The degradation efficiency of mixed substrates was slightly higher than single substrate.

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Available abstract

By gradually increasing the concentration of BTEX in the culture medium with toluene,benzene and xylene as the sole carbon source,a BTEX-degrading fungi strain which was designated as B1 was isolated from the sludge of sewage treatment plant.Using single factor and orthogonal experiment analyzed and studied the fungal degradation environment effect factors and degradation efficiency.The results showed that the optimum condition of fungal degradation was C∶N = 5∶1,pH5,temperature 30 ℃,strains inoculation amount 5.5ml(50ml culture medium).GC analysis was used to determine the fungal degradation effect to BTEX with the initial liquid phase concentration of 0~90mg/L,the results showed that the degradation activities of fungal strain was not inhibited,and the fungal degradation efficiency was toluene benzene xylene,the highest degradation efficiency reached 87.39%,85.21%,81.47%,respectively.The degradation efficiency of mixed substrates was slightly higher than single substrate.

Key concepts: BTEX, Degradation (telecommunications), Toluene, Benzene, Xylene, Strain (injury), Chemistry, Biodegradation

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