The effects of superovulation and strains on 2-cell mouse embryos culture in vitro.

Objective:Compared the methods of superovulation and the development of KM mouse and ICR mouse 2-cell embryos.Methods:29 KM mice were divided into two groups,after 48h or 72h of given PMSG,hCG was injected,and the 2-cell embryos were cultured in vitro.After that,ICR mouse and KM mouse were superovulated according to the conventional method,and the 2-cell embryos were cultured for 72h.Results:The numbers of KM mouse embryos and the percentage of KM mouse blastocysts were significant difference between 48h and 72h(P0.05).The group of 48h had the more number of embryos but the lower percentage of blastocysts.The difference of the percentage of blastocysts between KM mouse and ICR mouse wasn′t significance(P0.05).Conclusion:The interval of injected PMSG and HCG will affect the number and the development of mouse embryos.The development in vitro of 2-cell embryos is same between KM mouse and ICR mouse.