Determination of evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth by HPLC

AIM: To establish a method for determination of evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth by HPLC. METHODS : Evodiamine and rutaecarpine were extracted with the solvent ethyl acetate and the ultrasonic extractor. The separation was performed on YWG-C_ 18 (4.6mm×250mm,10μm) column with water-methanol-acetonitrile (42∶20∶38) as mobile phase. The flow rate was 1.0ml·min -1 , the column temperature was at 30℃ and the wavelength of UV detector was at 260nm. RESULTS : For evodiamine,the regression equation was Y=56.47X+29.89,r =0.9997, the linar range was within 5～1000μg and the recovery rate was 99.83%. the inter-day and intra-day precision (RSD) were 0.85% and 1.40%,respectively. For rutaecarpine,the regression equation was Y=49.08X+27.10,r= 0.9999,the linear range was within 4.5 ～900μg,the recovery rate was 98.72%. the inter-day and intra-day precision (RSD) were 0.63% and 1.17% ,respectively. The contents of evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth were 0.387% and 0.309% respectively. CONCLUSION : The method for determination of evodiamine and rutaecarpine by HPLC is simple,accurate,rapid,sensitive and with good reproducibility. It is a better method to determine evodiamine and rutaecarpine in Evodia rutaecarpa (Juss.) Benth.