Determination of Rutaecarpine and Evodiamine in Evodia Rutaecarpa by Supercritical Fluid Chromatography

A supercritical fluid chromatographic(SFC) method for the determination of rutaecarpine and evodiamine in Evodia rutaecarpa was established.The separation of rutaecarpine and evodiamine was performed on a Kromasil Slica column(250 mm×4.6 mm,5 μm) using CO2 containing 13% methanol as mobile phase at a flow rate of 2.0 mL·min-1.The back pressure was set at 2.0×107 Pa and the column temperature was 60 ℃.The detection wavelength was 225 nm.The result indicated that the linear ranges of rutaecarpine and evodiamine were in the range of 0.33-8.25 μg(r=0.999 3) and 0.68-17 μg(r= 0.999 8),respectively.The average recoveries were both 100% with RSD of 2.6% for rutaecarpine and 3.1% for evodiamine,respectively.Comparing with the traditional method,the proposed method was simple,rapid and sensitive,and could be successfully applied in the separation of rutaecarpine and evodiamine.