THE ANTITUMOR EFFECT AND MECHANISM OF SODIUM SELENITE ON HUMAN GASTRIC CANCER CELL LINES BGC823 IN VITRO

Objective To investigate the inhibitory effect of sodium selenite on human gastric cancer cell lines BGC823 and its possible mechanism.Methods The inhibitory effects of sodium selenite with different concentrations(0、4.0、8.0、10.0μmol/L) on BGC823 cells were detected by MTT assay.The cell cycle distribution was measured by flow cytometry(FCM).The apoptosis was examined by AnnexinV-FITC/PI staining.The apoptosis morphology was observed by fluorescence microscope after DAPI staining.FCM was used to quantitate the expression of FAS.Results MTT assay showed that sodium selenite inhibited the growth of BGC823 gastric cancer cells dose-dependently.Sodium selenite blocked BGC823 cells in S phase and increased the apoptosis rates of BGC823 cells dose-dependently.The typical apoptotic morphological changes including cell nucleus shrinkage,chromatin condensation and apoptotic bodies were observed when treated with different doses of sodium selenite.FCM showed that sodium selenite increased expressions of FAS dose-dependently.Conclusion Sodium selenite could inhibit human BGC823 gastric cancer cells proliferation and block cell cycle in S phase and induce apoptosis.The upregulation of FAS protein might be one of the mechanisms.[ACTA NUTRIMENTA SINICA,2011,33(6):571-574,579]